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magnetic labeling with anti cd1a  (Miltenyi Biotec)


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    Miltenyi Biotec magnetic labeling with anti cd1a
    Magnetic Labeling With Anti Cd1a, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+cd1a+microbeads/CD1a+MicroBeads%2C+human/bio_rxiv__64898__2026__05__05__723095-237-13-20
    Average 93 stars, based on 43 article reviews
    magnetic labeling with anti cd1a - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    Isolation:

    Article Title: Single-cell sequencing of human Langerhans cells identifies altered gene expression profiles in patients with atopic dermatitis.
    Article Snippet: The single-cell suspension was resuspended extensively, filtered, and layered onto a Ficoll (STEMCELL Technologies) gradient. .. LCs were isolated from the Ficoll fraction using human CD1a MicroBeads in magneticactivated cell sorting (Miltenyi Biotec). ..

    Article Title: Single-cell sequencing of human Langerhans cells identifies altered gene expression profiles in patients with atopic dermatitis
    Article Snippet: The single-cell suspension was resuspended extensively, filtered, and layered onto a Ficoll (STEMCELL Technologies) gradient. .. LCs were isolated from the Ficoll fraction using human CD1a MicroBeads in magnetic-activated cell sorting (Miltenyi Biotec). ..

    Article Title: Local accumulation of FOXP3+ regulatory T cells: evidence for an immune evasion mechanism in patients with large condylomata acuminata.
    Article Snippet: .. T cells were isolated on a T cell enrichment column (R&D Systems); LCs were isolated from the left cells with human CD1a MicroBeads (Miltenyi Biotec); the final cells were used for the isolation of macrophages by human CD11b MicroBeads (Miltenyi Biotec). .. In addition, normal PBMCs were used for the isolation of Treg cells with the Regulatory T Cell Isolation Kit (Miltenyi Biotec).

    FACS:

    Article Title: Single-cell sequencing of human Langerhans cells identifies altered gene expression profiles in patients with atopic dermatitis.
    Article Snippet: The single-cell suspension was resuspended extensively, filtered, and layered onto a Ficoll (STEMCELL Technologies) gradient. .. LCs were isolated from the Ficoll fraction using human CD1a MicroBeads in magneticactivated cell sorting (Miltenyi Biotec). ..

    Magnetic Cell Separation:

    Article Title: Single-cell sequencing of human Langerhans cells identifies altered gene expression profiles in patients with atopic dermatitis
    Article Snippet: The single-cell suspension was resuspended extensively, filtered, and layered onto a Ficoll (STEMCELL Technologies) gradient. .. LCs were isolated from the Ficoll fraction using human CD1a MicroBeads in magnetic-activated cell sorting (Miltenyi Biotec). ..



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    magnetic labeling with anti cd1a  (Miltenyi Biotec)
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    Miltenyi Biotec magnetic labeling with anti cd1a
    Magnetic Labeling With Anti Cd1a, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    cd1a microbeads  (Miltenyi Biotec)
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    Characterization of ImmuSkin-MT, ImmuSkin-M, and ImmuSkin-T. Skin models were subjected to H&E and immunofluorescence staining against loricrin as a marker for differentiated keratinocytes (red) and <t>CD1a</t> as a specific DC marker (green). DAPI (blue) was used as a nucleus counter-staining. Carboxyfluorescein succinimidyl ester (CFSE, green) is an indicator of T-lymphocytes indicated the absence of T-cells in the epidermis, dermis, and the immune layer. The white dotted line indicates the boundary between epidermis and dermis. In some pictures from immunofluorescence staining, a membrane of the transwell insert is presented in a bright yellow-green strip. Images were captured at a magnification of 20x. Scale bar = 100 µm. n = 3. The schematic part of this figure was created with BioRender.com
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    human cd1a microbeads  (Miltenyi Biotec)
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    Miltenyi Biotec human cd1a microbeads
    Characterization of ImmuSkin-MT, ImmuSkin-M, and ImmuSkin-T. Skin models were subjected to H&E and immunofluorescence staining against loricrin as a marker for differentiated keratinocytes (red) and <t>CD1a</t> as a specific DC marker (green). DAPI (blue) was used as a nucleus counter-staining. Carboxyfluorescein succinimidyl ester (CFSE, green) is an indicator of T-lymphocytes indicated the absence of T-cells in the epidermis, dermis, and the immune layer. The white dotted line indicates the boundary between epidermis and dermis. In some pictures from immunofluorescence staining, a membrane of the transwell insert is presented in a bright yellow-green strip. Images were captured at a magnification of 20x. Scale bar = 100 µm. n = 3. The schematic part of this figure was created with BioRender.com
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    Image Search Results


    Characterization of ImmuSkin-MT, ImmuSkin-M, and ImmuSkin-T. Skin models were subjected to H&E and immunofluorescence staining against loricrin as a marker for differentiated keratinocytes (red) and CD1a as a specific DC marker (green). DAPI (blue) was used as a nucleus counter-staining. Carboxyfluorescein succinimidyl ester (CFSE, green) is an indicator of T-lymphocytes indicated the absence of T-cells in the epidermis, dermis, and the immune layer. The white dotted line indicates the boundary between epidermis and dermis. In some pictures from immunofluorescence staining, a membrane of the transwell insert is presented in a bright yellow-green strip. Images were captured at a magnification of 20x. Scale bar = 100 µm. n = 3. The schematic part of this figure was created with BioRender.com

    Journal: Archives of Toxicology

    Article Title: A hair-follicle reconstructed in vitro immunocompetent skin model for prediction of the sensitizing potential of chemicals

    doi: 10.1007/s00204-025-04130-z

    Figure Lengend Snippet: Characterization of ImmuSkin-MT, ImmuSkin-M, and ImmuSkin-T. Skin models were subjected to H&E and immunofluorescence staining against loricrin as a marker for differentiated keratinocytes (red) and CD1a as a specific DC marker (green). DAPI (blue) was used as a nucleus counter-staining. Carboxyfluorescein succinimidyl ester (CFSE, green) is an indicator of T-lymphocytes indicated the absence of T-cells in the epidermis, dermis, and the immune layer. The white dotted line indicates the boundary between epidermis and dermis. In some pictures from immunofluorescence staining, a membrane of the transwell insert is presented in a bright yellow-green strip. Images were captured at a magnification of 20x. Scale bar = 100 µm. n = 3. The schematic part of this figure was created with BioRender.com

    Article Snippet: On day 6, CD1a + MoLCs were magnetically labeled and isolated using CD1a MicroBeads, human (Miltenyi Biotech, Bergisch Gladbach, Germany).

    Techniques: Immunofluorescence, Staining, Marker, Membrane, Stripping Membranes

    Influence of cocultivation on the migration and maturation of MoLCs and the proliferation of T-cells. Cells harvested from the lower chamber of each ImmuSkin variant were subjected to flow cytometry analysis and stained with anti-CD1a-, anti-CD86- and anti CD4-antibodies. a Representative dot plots show the populations of T-lymphocytes and MoLCs from each ImmuSkin type. The upper left side of the quadrant represents T-lymphocytes, while MoLCs are shown in the lower right side of the quadrant. Moreover, MoLC migration ( b ), MoLC maturation ( c ) and T-cell proliferation ( d ) were measured. The black bar represents immune cells from ImmuSkin-M, the striped bar represents immune cells from ImmuSkin-T, while the gray bar represents immune cells from ImmuSkin-MT. Data are presented as mean ± SD, and statistical significance is indicated as * p -value ≤ 0.05, ** p -value ≤ 0.01, *** p -value ≤ 0.001. A paired t-test was used for the statistical analysis. n = 3. The schematic part of this figure was created with BioRender.com

    Journal: Archives of Toxicology

    Article Title: A hair-follicle reconstructed in vitro immunocompetent skin model for prediction of the sensitizing potential of chemicals

    doi: 10.1007/s00204-025-04130-z

    Figure Lengend Snippet: Influence of cocultivation on the migration and maturation of MoLCs and the proliferation of T-cells. Cells harvested from the lower chamber of each ImmuSkin variant were subjected to flow cytometry analysis and stained with anti-CD1a-, anti-CD86- and anti CD4-antibodies. a Representative dot plots show the populations of T-lymphocytes and MoLCs from each ImmuSkin type. The upper left side of the quadrant represents T-lymphocytes, while MoLCs are shown in the lower right side of the quadrant. Moreover, MoLC migration ( b ), MoLC maturation ( c ) and T-cell proliferation ( d ) were measured. The black bar represents immune cells from ImmuSkin-M, the striped bar represents immune cells from ImmuSkin-T, while the gray bar represents immune cells from ImmuSkin-MT. Data are presented as mean ± SD, and statistical significance is indicated as * p -value ≤ 0.05, ** p -value ≤ 0.01, *** p -value ≤ 0.001. A paired t-test was used for the statistical analysis. n = 3. The schematic part of this figure was created with BioRender.com

    Article Snippet: On day 6, CD1a + MoLCs were magnetically labeled and isolated using CD1a MicroBeads, human (Miltenyi Biotech, Bergisch Gladbach, Germany).

    Techniques: Migration, Variant Assay, Flow Cytometry, Staining